Optimization of CTAB-based RNA Extraction Method for Aspergillus flavus
نویسندگان
چکیده
منابع مشابه
TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies
RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. Although many RNA extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. Many studies have shown that TRIzol is an applicable material for the RNA extracti...
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Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...
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Aspergillus flavus is one of the most important producers of carcinogenic aflatoxins in crops, and the effect of water activity (a(w)) on growth and aflatoxin production of A. flavus has been previously studied. Here we found the strains under 0.93 a(w) exhibited decreased conidiation and aflatoxin biosynthesis compared to that under 0.99 a(w). When RNA-Seq was used to delineate gene expression...
متن کاملNitrification by Aspergillus flavus.
Marshall, K. C. (Cornell University, Ithaca, N. Y.) and M. Alexander. Nitrification by Aspergillus flavus. J. Bacteriol. 83:572-578. 1962.-Aspergillus flavus has been shown to produce bound hydroxylamine, nitrite, and nitrate when grown in peptone, amino acid, or buffered ammonium media. Free hydroxylamine was not detected in these cultures, but it was found in an unbuffered ammonium medium in ...
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Aflatoxins are highly toxic cancer causing fungal metabolites known to cause immune-system suppression, growth retardation, liver disease, and death in both humans and domestic animals. Human exposure to aflatoxins is limited by regulations that prohibit the use of crops containing excess quantities of aflatoxins for foods and feeds. Aflatoxins are regulated in part per billion (ppb) ranges wit...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2020
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2020.908.038